Hi PGers and Jeremy,
I have watched this video and it is very informative even though I am a bit puzzled.
My impression was that once the mantle piece and the nucleus are implanted, the pearl sac is formed and as I understand that the Akoyas are being killed through the harvesting process, it is not possible for them to have a pearl sac ready for a second operation but in the video I hear that an incision is made into the (already existing) pearl sac and the nucleus plus mantle piece are put into place (I think it is close to the gonad). As I have understood it though, the pearl sac starts forming after the epithelial cells come into contact within the oyster.
Otherwise excellent information, strongly reminding of PP:s own video.